酵母选择营养缺陷培养基
酵母培养基配制Yeast media preparation
【液体培养基】 8g粉状培养基Cenvathys目录号CVAA013系列选择培养基加950 ml 水,常规高压灭菌后再加50ml 40%过滤除菌的无菌葡萄糖。（备注： YPD、YPDA和YPD plus这三种系列不需要再加糖，直接按瓶子上标签指示加水配制即可）。配制液体培养基不需要调pH，因为酵母偏好酸性条件生长。如果为了实验方便，葡萄糖也可按2%的量以粉末的形式加进去高压灭菌（见下面标准配制法注解）。
【固体培养基】8g粉状培养基(Cenvathys目录号CVAA013)加950 ml, 用NaOH调pH至大致范围5.8~6.5, 然后按20g/L加琼脂粉, 高压灭菌后再加预温的50ml 40%无菌葡萄糖, 摇匀后倾倒平皿(备注:如不调pH, 琼脂在酸性条件下会产生崩解)。如果为了实验方便，葡萄糖也可按2%的量以粉末的形式加进去高压灭菌。
【标准配制法】标准流程要求葡萄糖过滤除菌，因为葡萄糖与培养基中的氨基酸在高温高压下发生化学反应。但有些实验者为方便起见，将固体葡萄糖按2% 的量(按每升20克)直接加到培养基后一起高压灭菌，虽然酵母也可以生长，但一般不推荐这种将葡萄糖高压灭菌的方法。如果实在要图省事想用这种方法，那么一定要控制好高压时间在18-20分钟结束，待压力下降和温度降到安全范围后应尽快取出。否则液体颜色会变成黄色而对酵母特性发生不可预知的影响。但半乳糖和棉子糖不能高压灭菌，只能用过滤除菌制备，建议制备高浓度40%的储存液，按计算好的体积加到高压灭菌的培养基使得终浓度为2%。40％无菌棉子糖和半乳糖可以通过当地经销商从Cenvathys公司订购。
【说明】用于组成型表达（如酵母双杂交、酵母单杂交）以及其它采用组成型启动子的实验一般都加葡萄糖；诱导型表达如采用GAL1启动子载体的表达一般都加半乳糖和棉子糖。葡萄糖可以勉强高压灭菌，但半乳糖和棉子糖是绝对不能高压灭菌的, 应采用无菌过滤法先制备40%的无菌储备液，待不含糖的培养基高压灭菌后按最终应用浓度为2％ 的量计算需要加入的无菌液体糖储存液：CVAA013-X系列酵母选择营养缺陷培养基Yeast selective media or selective minus media
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货号 |
品名 |
包装 |
价格 |
|
CVAA013-01 |
SC Complete medium |
40g(8g/L) |
630.00 |
|
CVAA013-2 |
-Trp |
40g(8g/L) |
630.00 |
|
CVAA013-3 |
-Ura |
40g(8g/L) |
630.00 |
|
CVAA013-4 |
-His |
40g(8g/L) |
630.00 |
|
CVAA013-5 |
-Leu |
40g(8g/L) |
630.00 |
|
CVAA013-6 |
-His-Leu |
40g(8g/L) |
630.00 |
|
CVAA013-7 |
-Trp-Leu |
40g(8g/L) |
630.00 |
|
CVAA013-8 |
-Trp-Leu-His |
40g(8g/L) |
630.00 |
|
CVAA013-9 |
-Trp-Leu-His-Ade |
40g(8g/L) |
630.00 |
|
CVAA013-10 |
-Trp-Leu-His-Ura |
40g(8g/L) |
630.00 |
|
CVAA013-11 |
-Trp-Leu-His-Ura-Ade |
40g(8g/L) |
630.00 |
|
CVAA013-12 |
-Trp-Ade |
40g(8g/L) |
630.00 |
|
CVAA013-13 |
-Ura-His |
40g(8g/L) |
630.00 |
|
CVAA013-14 |
-Ade |
40g(8g/L) |
630.00 |
|
CVAA013-15 |
-Trp-His-Ura |
40g(8g/L) |
630.00 |
|
CVAA013-16 |
-Leu-His-Ura |
40g(8g/L) |
630.00 |
|
CVAA013-17 |
-Trp-His |
40g(8g/L) |
630.00 |
|
CVAA013-18 |
-Trp-His-Ade |
40g(8g/L) |
630.00 |
|
CVAA013-19 |
-Trp-Leu-Ura |
40g(8g/L) |
630.00 |
|
CVAA013-20 |
-Trp-Leu-Ade |
40g(8g/L) |
630.00 |
|
CVAA013-21 |
-Leu-His-Ade |
40g(8g/L) |
630.00 |
|
CVAA013-22 |
-Ura-Ade |
40g(8g/L) |
630.00 |
|
CVAA013-23 |
-His-Ade |
40g(8g/L) |
630.00 |
|
CVAA013-24 |
-Leu-Ade |
40g(8g/L) |
630.00 |
|
CVAA013-31 |
-Trp-Ura |
40g(8g/L) |
630.00 |
|
CVAA013-32 |
-Trp-Ura-Ade |
40g(8g/L) |
630.00 |
|
CVAA013-35 |
-Leu-Ura |
40g(8g/L) |
630.00 |
|
CVAA013-36 |
-Leu-Ura-Ade |
40g(8g/L) |
630.00 |
|
CVAA013-40 |
-Trp-Leu-His-Ade-Ura |
40g(8g/L) |
630.00 |
|
CVAA013-50 |
YPD |
250g(50g/L) |
520.00 |
|
CVAA013-51 |
YPD plus |
250g(50g/L) |
520.00 |
|
CVAA013-52 |
YPDA |
250g(50g/L) |
520.00 |
|
CVAA013-60 |
-Trp-Leu-His-Ade-Ura-Met |
40g(8g/L) |
650.00 |
|
CVAA013-60Lys |
-Trp-Leu-His-Ade-Ura-Met-Lys |
40g(8g/L) |
650.00 |
|
CVAA013-61 |
-Met |
40g(8g/L) |
650.00 |
|
CVAA013-61T |
-Met-Trp |
40g(8g/L) |
650.00 |
|
CVAA013-61L |
-Met-Leu |
40g(8g/L) |
650.00 |
|
CVAA013-61H |
-Met-His |
40g(8g/L) |
650.00 |
|
CVAA013-61A |
-Met-Ade |
40g(8g/L) |
650.00 |
|
CVAA013-61U |
-Met-Ura |
40g(8g/L) |
650.00 |
|
CVAA013-61LC |
-Met-Leu-Cys |
40g(8g/L) |
750.00 |
|
CVAA013-62UC |
-Met-Ura-Cys |
40g(8g/L |
750.00 |
|
CVAA013-62TL |
-Met-Trp-Leu |
40g(8g/L) |
650.00 |
|
CVAA013-62TH |
-Met-Trp-His |
40g(8g/L) |
650.00 |
|
CVAA013-62TA |
-Met-Trp-Ade |
40g(8g/L) |
650.00 |
|
CVAA013-62TU |
-Met-Trp-Ura |
40g(8g/L) |
650.00 |
|
CVAA013-62LH |
-Met-Leu-His |
40g(8g/L) |
650.00 |
|
CVAA013-62LA |
-Met-Leu-Ade |
40g(8g/L) |
650.00 |
|
CVAA013-62LU |
-Met-Leu-Ura |
40g(8g/L) |
650.00 |
|
CVAA013-62UA |
-Met-Ura-Ade |
40g(8g/L) |
650.00 |
|
CVAA013-63TLH |
-Met-Trp-Leu-His |
40g(8g/L) |
650.00 |
|
CVAA013-63TLA |
-Met-Trp-Leu-Ade |
40g(8g/L) |
650.00 |
|
CVAA013-63LHA |
-Met-Leu-His-Ade |
40g(8g/L) |
650.00 |
|
CVAA013-63LHU |
-Met-Leu-His-Ura |
40g(8g/L) |
650.00 |
|
CVAA013-63HAU |
-Met-His-Ade-Ura |
40g(8g/L) |
650.00 |
|
CVAA013-64TLHA |
-Met-Trp-Leu-His-Ade |
40g(8g/L) |
650.00 |
|
CVAA013-64TLHU |
-Met-Trp-Leu-His-Ura |
40g(8g/L) |
650.00 |
|
CVAA013-64TLAU |
-Met-Trp-Leu-Ade-Ura |
40g(8g/L) |
650.00 |
|
CVAA013-64LHAU |
-Met-Leu-His-Ade-Ura |
40g(8g/L) |
650.00 |
|
CVAA013-64THAU |
-Met-Trp-His-Ade-Ura |
40g(8g/L) |
650.00 |
|
CVAA013-65 |
-Lys |
40g(8g/L) |
750.00 |
|
CVAA013-66 |
-Arg |
40g(8g/L) |
750.00 |
|
CVAA013-67 |
-Ser |
40g(8g/L) |
750.00 |
|
CVAA013-68 |
-Thr |
40g(8g/L) |
750.00 |
|
CVAA013-69 |
-Lys-Arg-Ser-Thr |
40g(8g/L) |
750.00 |
|
CVAA013-70 |
-Arg-Lys |
40g(8g/L) |
750.00 |
|
CVAA013-71 |
-Ser-Thr |
40g(8g/L) |
750.00 |
|
CVAA013-72 |
-Arg-His-Leu-Ura |
40g(8g/L) |
750.00 |
|
CVAA013-73 |
-Trp-Lys |
40g(8g/L) |
750.00 |
|
CVAA013-74 |
-Arg-His-Leu |
40g(8g/L) |
750.00 |
|
CVAA013-75 |
-Arg-Leu-Ura |
40g(8g/L) |
750.00 |
|
CVAA013-76 |
-Lys-Ura |
40g(8g/L) |
750.00 |
|
CVAA013-77 |
-Ile-Val |
40g(8g/L) |
750.00 |
|
CVAA013-78 |
-Val |
40g(8g/L) |
750.00 |
|
CVAA013-79 |
-Ile |
40g(8g/L) |
750.00 |
|
CVAA013-80 |
-Ile-Val-Leu |
40g(8g/L) |
750.00 |
|
CVAA013-81 |
-Tyr |
40g(8g/L) |
750.00 |
|
CVAA013-82 |
-Asp |
40g(8g/L) |
750.00 |
|
CVAA013-83 |
-Phe |
40g(8g/L) |
750.00 |
|
CVAA013-84 |
-Arg-Ura-His |
40g(8g/L) |
750.00 |
|
CVAA013-85 |
-Arg-Leu |
40g(8g/L) |
750.00 |
|
CVAA013-86 |
-Ser-Trp-Leu |
40g(8g/L) |
750.00 |
|
CVAA013-87 |
-Lys-Leu-Ura |
40g(8g/L) |
750.00 |
|
CVAA013-88 |
-Lys-Arg-Leu-Ura-His |
40g(8g/L) |
750.00 |
|
CVAA013-89 |
-Lys-Leu-Ura-His |
40g(8g/L) |
750.00 |
|
CVAA013-90 |
-Lys-His |
40g(8g/L) |
750.00 |
|
CVAA013-91 |
-Ser-Trp-Leu-Ade |
40g(8g/L) |
750.00 |
|
And more……… |
more……… |
40g/L |
more….. |
|
CVAA013-N |
Elements Minus/selective |
200g/L |
2440-2520 |
|
CVAA013C-1 |
3-AT |
5ml sterile |
|
|
CVAA013C-2 |
5-FOA |
250/500mg |
|
|
CVAA013D-1 |
40%无菌葡萄糖 |
Discontinued |
|
|
CVAA013D-2 |
40%无菌半乳糖 |
50ml |
|
|
CVAA013D-3 |
40%无菌棉籽糖（棉子糖） |
50ml |
|
| CVAA013E |
Yeast Transformation Kit |
20T/Kit |
|
| CVAA013F |
Yeast Plasmid Isolation Kit |
20T/Kit |
|
| CVAA013G |
Yeast Protein Isolation Kit |
20T/Kit |
|
| CVAA013H |
10xLiAc无菌 |
20ml |
|
| CVAA013I |
50%PEG无菌 |
20ml |
|
| CVAA013J |
Carrier DNA 运载DNA 酵母转化 |
20ml |
|
| CVAA013K |
10xTE 无菌 |
20ml |
|
| CVAA013L |
Beta-Gal |
20mg/ml |
|
