Mouse interleukin 10,IL-10 ELISA KIT

产品详情

• 产品描述：

  The product CSB-E04594m is a ready-to-use microwell, strip plate ELISA kit for quantitatively analyzing the presence of the IL-10 in different biological samples, including serum, plasma, cell culture supernates, and tissue homogenates. This IL-10 ELISA kit specifically recognizes the mouse IL-10 in the sample. It adopts the Sandwich ELISA technique in conjunction with antibody-antigen specific binding and an enzyme colorimetric reaction to indicate the presence of mouse IL-10 and quantitatively measure the concentration of IL-10 in the samples.

  IL-10 is an immunosuppressive cytokine that predominantly exerts an anti-inflammatory effect. IL-10 mainly counters the synthesis of inflammatory mediators, particularly in response to TLR signaling. The IL-10/STAT3/SOCS3 signaling is involved in critical cellular processes such as cell growth, apoptosis, and transcription of inflammatory genes.

• 别名：

  Il10 ELISA Kit; Il-10Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit

• 缩写：

  IL10

• Uniprot No.：

  P18893

• 种属：

  Mus musculus (Mouse)

• 样本类型：

  serum, plasma, tissue homogenates

• 检测范围：

  62.5 pg/ml - 4000 pg/ml

• 灵敏度：

  15.6 pg/ml

• 反应时间：

  1-5h

• 样本体积：

  50-100ul

• 检测波长：

  450 nm

• 研究领域：

  Immunology

• 测定原理：

  quantitative

• 测定方法：

  Sandwich

• 说明书：

  下载最新说明书

• 本试剂盒所含材料：

  • • A micro ELISA plate --- The 96-well plate has been pre-coated with anti-mouse IL-10 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • Biotin-labeled IL-10 antibody (100 x concentrate) 1 x 120 μl ---Act as the detection antibody.
    • HRP-avidin (100 x concentrate) 1 x 120 μl --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • Biotin-antibody Diluent 1 x 15 ml ---Dilute the high concentration Biotin-antibody to an appropriate working solution.
    • HRP-avidin Diluent 1 x 15 ml ---Dilute the high concentration HRP-avidin solution to an appropriate solution.

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• 本试剂盒不含材料：

  • • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate